Figure 6.
Kinetics of the recruitment of transcription factors directed by apo-ERα isoforms on the pS2 promoter. (A, B) Kinetic ChIP experiments were performed and expressed as in Figure 4, using antibodies and chromatin prepared from synchronized MDA-MB231 cells expressing either ERα isoforms, as indicated within the graphs. (C) Scheme summarizing the sequence of recruitment of transcription factors directed by ERα66 and ERα46 onto the pS2 promoter. The numbering reflects the order of engagement of the different complexes onto the promoter. In the case of ERα46, V-a and V-b are combinatorial steps.