Table 3. Comparison of the activity of some hit compounds at 20 μM and 50 μM against stationary phase B. burgdorferia.
NSC number | Compounds | Residual viable cells (%) | ||
---|---|---|---|---|
20 μMb | 20 μMc | 50 μMc | ||
258812 | Control | 93 | 94 | 95 |
Amoxicillin | 77 | 77 | 77 | |
Doxycycline | 76 | 77 | 77 | |
Daptomycin | 32 | 25 | 18 | |
Dimethyldaunomycin hydrochloride | 0 | 10 | 10 | |
363998 | Anthracene-9,10-dione, 1,5-bis[3-[[(2-hydroxyethyl)amino] propyl]amino]-9,10-dihydro-, dihydrochloride | 22 | 14 | 13 |
70845 | Nogalamycin | 3 | 15 | 15 |
267229 | Pyrromycin | 6 | 20 | 21 |
136044 | Rhodomycin A | 5 | 21 | 21 |
345647 | Chaetochromin | 31 | 33 | 22 |
47147 | Prodigiosin | 0 | 45 | 24 |
267461 | Nanaomycin | 39 | 45 | 26 |
659997 | Naphthalene-1,4-dione, 2-chloro-5,8-dihydroxy-3-(2-methoxyethoxy)- | 40 | 50 | 28 |
224124 | (5,8-dihydroxy-1,4-dioxo-1,4-dihydronaphthalene-2,3-diyl)dimethanediyl dicarbamate | 54 | 77 | 31 |
617570 | Benzoic acid, 2-hydroxy-, (2,6-pyridinediyldiethylidyne) dihydrazide, nickel complex | 66 | 50 | 38 |
93739 | 2-Methyl-4,4′-[(4-imino-2,5-cyclohexadien-1-ylidene)methylene]dianiline hydrochloride | 0 | 77 | 43 |
Seven-day-old stationary phase B. burgdorferi culture was treated with drugs for seven days.
Residual viable B. burgdorferi was calculated according to the regression equation and the ratio of Green/Red fluorescence obtained by the SYBR Green I/PI assay.
Residual viable B. burgdorferi was assayed by epifluorescence microscope counting.