Table 1.

Primers and PCR temperature profile used in this study.

Primers	Sequences (5′–3′)	Target position	PCR temperature profiles	References
nifHf	TACGGNAARGGSGGNATCGGCAA	nifH (28–51)	95 °C 5 min, 20X (94 °C 30 s, 65 °C decrease on 0.5 °C in each cycle 30s, 72 °C 1.5 min), 25X (94 °C 30 s, 55 °C 30s, 72 °C 1.5 min) 72 °C 10 min	Nzoué et al. (2009)
nifHr	AGCATGTCYTCSAGYTCNTCCA	nifH(809–787)
glnIIf	AAGCTCGAGTACATCTGGCTCGACG	glnII (13–38)	95 °C 2 min, 35X (95 °C 45 s, 65 °C 30s, 72 °C 1.5 min) 72 °C 10 min	Stępkowski et al. (2011)
glnIIr	SGAGCCGTTCCAGTCGGTGTCG	glnII (681–660)