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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jan 14;470(2):275–281. doi: 10.1016/j.bbrc.2016.01.071

Fig. 4.

Fig. 4

Senescent NSGCs enhance HBMEC growth and GSC-derived tumor formation. (A) ELISA of VEGFs in the conditioned medium (CM) of GBM146 and GBM157 cells precultured in serum-containing medium for the indicated time periods. For CM preparation, cells were cultured in serum-containing medium for 5 days, and the culture supernatants were collected. (B) ELISA of IL-6 and IL-8 in CM prepared as in A. (C) Promotion of HBMEC growth by the CM of senescent NSGCs (day 30 after serum exposure). Error bars, standard deviations. Statistical evaluations were performed using the Welch t test. **, P < 0.001. (D) Promotion of GSC-derived tumor formation by senescent but not differentiated NSGCs. GSCs with or without differentiated/senescent NSGCs were injected subcutaneously into NOD/SCID mice. After three months, tumor formation and its volume were evaluated.