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. 2016 Aug 3;95(4):81. doi: 10.1095/biolreprod.116.141770

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© 2016 by the Society for the Study of Reproduction, Inc.

This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0

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FIG. 6 — MKI67 is not expressed in spermatogonia immediately following WIN 18,446/RA exposure. Images depicts representative cross sections of seminiferous tubules taken from mice treated with WIN 18,446 followed by an RA injection and allowed to recover for 16 h (A), 46 h (B), and 72 h (C) and stained for GCNA and MKI67. White arrows denote GCNA only-positive spermatogonia, orange arrows indicate MKI67 only-positive cells, and purple arrows depict GCNA- and MKI67-copositive spermatogonia. Bars = 25 μm. Bar graph indicates the average percentage of GCNA only-positive and GCNA- and MKI67-copositive germ cells per round tubule from testes of mice following synchronization, displayed in D. * denotes a P-value < 0.0005. Error bars represent SEM.