(A) PEO1 cells stimulated with or without IFNγ (20ng/L, 24h) were treated with or without 200 nM JQ1 for 72 hours. PD-L1 expression was determined by FACS.
(B) PEO1 cells with or without IFNγ (20ng/mL, 24h) stimulation were treated with indicated doses of JQ1 for 72 hours. PD-L1 expression was determined by FACS.
(C) PEO1 cells were treated with 200 nM JQ1. PD-L1 expression was determined by FACS at the indicated time points.
(D) PEO1 cells were treated with 200 nM JQ1. mRNA expression of CD274 (encoding PD-L1) was determined by qRT-PCR at the indicated time points.* p<0.05.
(E) PEO1 cells were treated with the indicated doses of JQ1 for 72 hours, and CD274 (encoding PD-L1) mRNA expression was determined by qRT-PCR. * p<0.05.
Error bars represent S.E.M. of three independent experiments.