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. Author manuscript; available in PMC: 2017 Sep 13.
Published in final edited form as: Cell Rep. 2016 Sep 13;16(11):2829–2837. doi: 10.1016/j.celrep.2016.08.032

Figure 2. BET inhibitor JQ1 suppresses PD-L1 expression at the transcriptional level. See also Figure S2.

Figure 2

(A) PEO1 cells stimulated with or without IFNγ (20ng/L, 24h) were treated with or without 200 nM JQ1 for 72 hours. PD-L1 expression was determined by FACS.

(B) PEO1 cells with or without IFNγ (20ng/mL, 24h) stimulation were treated with indicated doses of JQ1 for 72 hours. PD-L1 expression was determined by FACS.

(C) PEO1 cells were treated with 200 nM JQ1. PD-L1 expression was determined by FACS at the indicated time points.

(D) PEO1 cells were treated with 200 nM JQ1. mRNA expression of CD274 (encoding PD-L1) was determined by qRT-PCR at the indicated time points.* p<0.05.

(E) PEO1 cells were treated with the indicated doses of JQ1 for 72 hours, and CD274 (encoding PD-L1) mRNA expression was determined by qRT-PCR. * p<0.05.

Error bars represent S.E.M. of three independent experiments.