Table 2.

Quasispecies fluctuations and mutations during the transition from human tissue to Vero cell culture. Nucleotide variations observed between primary human tissue isolates and their respective subsequent Vero cell culture. Both quasispecies selection (SIN3275V → SIN849M) and new emergence (AS → HSR1) are observed during the transmission of SARS-CoV from human tissue sample into Vero cell culture.

Source of viral sequence: Human Tissue	Source of viral sequence: Passage to Vero cell culture	Nucleotide Heterogeneity	Position (Urbani)	ORF (based on NC_004718.3)
Sin3408L	Sin842M	-	-	-
Sin3725V	Sin849M	Y [t/C] → T	548	95aa of Leader protein (I, T → I)
		Y [T/C] → C	13347	131aa of NSP10 (SILENT)
Sin3765V	Sin852M	-	-	-
AS	HSR 1	G → R [G/A]	27254	637aa of sars6 (D → D, N)