Table 1.
Number of rats with low-, intermediate- and high-level POMC mRNA or protein expression in tanycytes in the different experiments. In each experiment, rats were euthanized within 2h of the mid-day period.
| Number of rats euthanized together | Sex | Weight (g) | Age | POMC mRNA or protein levels | ||
|---|---|---|---|---|---|---|
| Low | Intermediate | High | ||||
| Adult rats for Pomc ISH | ||||||
| 4 | M | 240-260 | ∼8 weeks | 3 | 1 | 0 |
| 6 | M | 257-284 | ∼8-9 weeks | 4 | 0 | 2 |
| 6 | F | 224-245 | ∼9-10 weeks | 1 | 2 | 3 |
| 4 | M | 286-293 | ∼9-10 weeks | 0 | 0 | 4 |
| 6 | M | 413-436 | 15 weeks | 2 | 2 | 2 |
| Adult rats for POMC immunofluorescence | ||||||
| 4 | M | 290-320 | ∼10 weeks | 1 | 1 | 2 |
| 4 | M | 290-320 | ∼10 weeks | 2 | 1 | 1 |
| 7 | F | 238-258 | 11 weeks | 5 | 1 | 1 |
| Total: 41 | 17 | 9 | 15 | |||
| Adolescent rats for Pomc ISH | ||||||
| 4 | M | 80-93 | 31 days | 4 | 0 | 0 |
| 4 | F | 67-81 | 31 days | 4 | 0 | 0 |