| Primary antibodies |
|
|
|
| POMC |
N-terminal 26 amino acids of porcine POMC: WCLESSQCQDLSTESNLLACIRACKP |
Phoenix Pharmaceuticals, H-029-30, RRID:AB_2307442 rabbit polyclonal |
1:20K for immunofluorescence 1:15K for electron microscopy |
| β-endorphin |
rat β-endorphin |
Phoenix Pharmaceuticals, H-022-33, RRID:AB_2314007 rabbit polyclonal |
1:10K |
| ACTH |
rat ACTH |
Phoenix Pharmaceuticals, H-001-21, RRID:AB_2572293 rabbit polyclonal |
1:10K |
| α-MSH |
α-MSH |
Millipore, AB5087, RRID:AB_91683 sheep polyclonal |
1:10K |
| vimentin |
recombinant vimentin |
Millipore, AB5733, RRID:AB_2216104 chicken polyclonal |
1:4K after ISH; 1:20K for immunofluorescence |
| HuC/HuD |
human HuC/D |
Life Technologies, A21271, RRID:AB_221448 mouse monoclonal |
2 μg/ml after ISH; 1 μg/ml for immunofluorescence |
| digoxigenin, peroxidase conjugated |
digoxigenin |
Roche, 11207733910, RRID:AB_514500 sheep |
1:100 |
| Secondary antibodies |
|
|
|
| Alexa 647- anti-mouse |
mouse IgG |
Jackson Immunoresearch, 715-605-151, RRID:AB_2340863 donkey polyclonal |
1:200 |
| Cy3- anti-chicken |
chicken IgY |
Jackson Immunoresearch, 703-165-155, RRID:AB_2340363 donkey polyclonal |
1:200 |
| Alexa 488- anti-rabbit |
rabbit IgG |
Jackson Immunoresearch, 711-545-152, RRID:AB_2313584 donkey polyclonal |
1:400 |
| Alexa 647-anti-chicken |
chicken IgY |
Jackson Immunoresearch, 703-605-155, RRID:AB_2340379 donkey polyclonal |
1:200 |
| Cy3- anti-mouse |
mouse IgG |
Jackson Immunoresearch, 715-165-151, RRID:AB_2315777 donkey polyclonal |
1:200 |
| Alexa 488- anti-sheep |
sheep IgG |
Jackson Immunoresearch, 713-545-147, RRID:AB_2340745 donkey polyclonal |
1:200 |
| Cy3- anti-rabbit |
rabbit IgG |
Jackson Immunoresearch, 711-165-152, RRID:AB_2307443 donkey polyclonal |
1:200 |
| Biotin-anti-rabbit |
rabbit IgG |
Jackson Immunoresearch, 711-065-152, RRID:AB_2340593 donkey polyclonal |
1:500 |
