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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 15;19(1):10.1111/cmi.12637. doi: 10.1111/cmi.12637

Figure 1. ORP1L localizes to the C. burnetii PV membrane.

Figure 1

Live cell confocal (A) or fixed immunofluorescence (B) microscopy images of uninfected or infected HeLa cells expressing ORP1L-GFP. In uninfected HeLa cells, ORP1L-GFP is found on vesicular structures (A, left). ORP1L-GFP also localizes to the PV membrane (A and B, arrows) in HeLa cells infected for three days with C. burnetii expressing red fluorescent protein mCherry. Cell boundaries are shown with dotted white lines. CD63 is a marker for the PV membrane in B. Scale bars = 10 µm.