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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Comp Neurol. 2016 Aug 18;525(3):535–552. doi: 10.1002/cne.24083

Figure 1.

Figure 1

Glial cells in longitudinal sections of P30 rat optic nerves.

A. Double labeled with rabbit anti-rat NG2 (green) and mouse CC1 antibody (magenta) that recognizes oligodendrocytes.

B. Double labeled with rabbit anti-rat NG2 (green) and guinea pig anti-GLAST (magenta) antibodies. NG2 cells and GLAST+ astrocytes represent non-overlapping cell populations.

C. Double labeled with rabbit anti-GFAP (green) and guinea pig anti-GLAST antibodies (magenta). GLAST and GFAP immunoreactivity are colocalized.

Scale bars = 20 µm. A: acquired by wide field microscopy. B and C: stacks of confocal images.