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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Comp Neurol. 2016 Aug 18;525(3):535–552. doi: 10.1002/cne.24083

Figure 7.

Figure 7

Quantification of nodes of Ranvier with axonal branchpoints in mouse thoracic ventral spinal cord white matter.

A–C: Quadruple immunostaining of P30 mouse spinal cord white matter sections labeled with AnkG (white), NG2 (green), GLAST (red), and Caspr (blue) antibodies. GLAST+ (astrocyte) and NG2+ cell processes contact the AnkG+ nodes, flanked by Caspr+ paranodes.

A: Confocal maximum projection of z-stack images, with an arrow indicating a node with a branchpoint leading to a third Caspr+ paranode. Scale bar, 5 µm.

B: Orthogonal views of the branchpoint node indicated in (A), showing astrocyte (GLAST, red) and NG2 cell (NG2, green) contact at the node (AnkG, white). Scale bar, 5 µm.

C: 3D volume rendering of the branchpoint node indicated in (A). Scale bar, 1 µm.

D, E: Differences in the mean nAD (D) and nGW (E) of all the nodes with or without an axonal branchpoint.

F, G: The mean nAD (F) and nGW (G) of nodes without branchpoints that had or did not have an NG2 cell process.

Mean ± SEM; ***, p < 0.001; **, p < 0.01; *, p < 0.05.