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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Comp Neurol. 2016 Aug 18;525(3):535–552. doi: 10.1002/cne.24083

Figure 8.

Figure 8

Pre-embedding immunoelectron microscopy of glial cells at the nodes.

A–C: Immunoperoxidase labeling using rabbit anti-NG2 antibody. A, B: P30 rat optic nerve; C: P30 mouse optic nerve. Slender immunolabeled NG2+ processes insert into the nodes obliquely and are directly apposed to a portion of the nodal axonal membrane.

D–F: Immunoperoxidase labeling using guinea pig anti-GLAST antibody. P30 rat optic nerve. GLAST+ processes appear as broad structures that cover the nodal membrane. In most cases, these processes do not seem to directly contact the axolemma.

Scale bars = 500 nm.