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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Comp Neurol. 2016 Aug 18;525(3):535–552. doi: 10.1002/cne.24083

Table 1.

Source, immunogen, and dilution of the primary antibodies used

Antibody
Name
Immunogen Manufacturer, RRID,
Host & Clonality
Dilution
AnkG full-length human Ankyrin G (~1000
amino acids)
UC Davis/NIH NeuroMab Facility
(Davis, CA), Cat# 73–146,
RRID: AB_10697718
mouse monoclonal antibody (clone N106/36)
1:50
Caspr QNHRYKGSYHTNEPKATHDSHPG
GKAPLPPSGPAQAPAPTPAPTQVPT
PAPAPASGPGPRDQNLPQILEESRS
UC Davis/NIH NeuroMab Facility
(Davis, CA), Cat# 73-001
RRID: AB_10671175
mouse monoclonal antibody (clone K65/35)
1:50
Caspr cytoplasmic domain of rat p190/
contactin-associated protein (Caspr)
Dr. Matthew Rasband (Baylor College of
Medicine, Houston, TX)
RRID: AB_2572297
rabbit polyclonal antibody
1:3000
CC1 amino acids 1–226 of human APC
(Ab-7) (CC-1)
Calbiochem (Millipore, Billerica, MA),
Cat#OP80
RRID: AB_2057371
mouse monoclonal antibody (clone CC1)
1:100
GFAP bovine glial fibrillary acidic protein
(GFAP)
Dako (Carpinteria, CA), Cat# Z0334
RRID: AB_10013382
rabbit polyclonal antibody
1:2000
GLAST QLIAQDNEPEKPVADSETKM
(c-terminus of GLAST)
Millipore (Billerica, MA), Cat# AB1782
RRID: AB_90959
guinea pig polyclonal antibody
1:1000
GLAST KNRDVEMGNSVIEENEMKKPYQLI
AQDNEPEKPVADSETKM
mouse GLAST, C-terminal 41 aa
Frontier Institute (Ishikari, Hokkaido, Japan),
Cat# GLAST-GP
RRID: AB_2571717
guinea pig polyclonal antibody
1:1000
NG2 extracellular domain of rat NG2, amino
acids 30–2225
Dr. William Stallcup
RRID: AB_2572299
guinea pig polyclonal antibody
1:1000
NG2 extracellular domain of rat NG2, amino
acids 30–2225
Dr. William Stallcup
RRID: AB_2572306
rabbit polyclonal antibody
1:1000
NG2 purified rat NG2 Chondroitin Sulfate
Proteoglycan
Millipore (Billerica, MA), Cat# AB5320
RRID: AB_91789
rabbit polyclonal antibody
1:500
Pan-
NaCh
CTEEQKKYYNAMKKLGSKK
(intracellular loop III-IV of sodium
channel α aubunit)
Sigma-Aldrich (St. Louis, MO), Cat# S8809
RRID: AB_477552
mouse monoclonal antibody (clone K58/35)
1:100