Table 2.
Normalization to the RPT internal standard improves accuracy in RIF quantification (n=5). Relative error was calculated using 1/x2 weighted linear regression. QC accuracy is reported as the average of the 5 trials using the difference from 100% of the absolute value of the percent error.
Concentration (μM) | Raw Relative Error (%) | Ratio Relative Error (%) |
---|---|---|
3.00 | 29.0 | 10.8 |
7.00 | 19.2 | 17.8 |
10.0 | 18.3 | 10.6 |
30.0 | 0.8 | 1.3 |
70.0 | 8.3 | 2.0 |
100.0 | 10.9 | 6.8 |
Concentration (μM) | Raw Accuracy (%) | Ratio Accuracy (%) |
---|---|---|
50.0 | 87.7 | 95.5 |