Skip to main content
. Author manuscript; available in PMC: 2017 Nov 12.
Published in final edited form as: Biofactors. 2016 Jun 21;42(6):703–715. doi: 10.1002/biof.1304

Figure 3. Expression of the Cxcl9, Cxcl10 and Cxcl11 genes is increased in a cytokine-dependent manner in rat β-cell lines.

Figure 3

A–C. 832/13 rat insulinoma cells were stimulated with 1 ng/mL IL-1β or IL-1β plus 100 U/mL IFN-γ for the indicated times (NT; no treatment). D–F. 832/13 cells were pretreated for 1 h with either DMSO or 0.5 μg/mL Cycloheximide (CHX). Cells were subsequently exposed to IL-1β (1 ng/mL) or the combination of IL-1β and IFN-γ (100 U/mL) for 2 h. Cellular mRNA levels of Cxcl9 (A, D), Cxcl10 (B, E) and Cxcl11 (C, F) were detected by RT-PCR. n.s. = not significant vs respective treatment in DMSO control group. Data are shown as means ± SEM from three independent experiments.