Table 3.
Competition of 125I-IDAPP binding in mouse brain
| Compound | Ki (nM) | Hill Slope |
|---|---|---|
| Morphine | 0.8 ± 0.2 | −0.6 ± 0.1 |
| Methadone | 1.4 ± 0.4 | −0.7 ± 0.1 |
| M6G | 0.7 ± 0.3 | −0.6 ± 0.1 |
| Buprenorphine | 0.2 ± 0.1 | −0.6 ± 0.1 |
| CTAP | 1.1 ± 0.3 | −0.5 ± 0.1 |
| DAMGO | 1.2 ± 0.5 | −0.9 ± 0.1 |
| Endomorphin 1 | 1.6 ± 0.5 | −0.7 ± 0.1 |
| Endomorphin 2 | 2.1 ± 0.8 | −0.5 ± 0.1 |
| SNC80 | >1,000 | |
| Naltrindole | >1,000 | |
| U50,488H | >1,000 | |
| NorBNI | >1,000 |
Competition assays were performed at 0.1 nM 125I-IDAPP in C57/Bl6 brain homogenates in the absence of blockers. Data represent mean ± SEM of at least 3 determinations of the Ki value. Ki values and Hill slopes were calculated using GraphPad Prism.