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. 2016 Dec;7(6):838–847. doi: 10.21037/jgo.2016.06.09

Table 1. Specifications of the reagents and antibodies used in this study.

Antibody Clone/code Company Dilution Antigen retrieval
EGFR D3881 Cell Signaling Technology Danvers, MA 1:100 Trilogy; water bath (95 °C 25 min)
HER2 E2-4001/Cat#AH01011 Invitrogen Camarillo, CA 1:400 Citra; steamer 30 min
HER3 2F12/Cat#05-390 EMD Millipore Billerica, MA 1:100 CC1 30 min
HER4 Cat#PA5-16789 Thermo Fisher Scientific Rockford, IL 1:100 Citra; steamer 30 min
cMet (Ventana) SP44/Cat#790-4430 Ventana Medical Systems Tucson, AZ Prediluted CC1 30 min
cMet (Leica) 8F11/NCL-cMet Leica Biosystems Newcastle, United Kingdom 1:80 Citra; steamer 30 min
Ki67 M1B-1/Cat#M7240 DAKO Carpinteria, CA 1:250 Trilogy; water bath (95 °C 25 min)

EGFR, epidermal growth factor receptor; HER, human epidermal growth factor receptor; cMet, hepatocyte growth factor receptor.