Table 1. Specifications of the reagents and antibodies used in this study.
Antibody | Clone/code | Company | Dilution | Antigen retrieval |
---|---|---|---|---|
EGFR | D3881 | Cell Signaling Technology Danvers, MA | 1:100 | Trilogy; water bath (95 °C 25 min) |
HER2 | E2-4001/Cat#AH01011 | Invitrogen Camarillo, CA | 1:400 | Citra; steamer 30 min |
HER3 | 2F12/Cat#05-390 | EMD Millipore Billerica, MA | 1:100 | CC1 30 min |
HER4 | Cat#PA5-16789 | Thermo Fisher Scientific Rockford, IL | 1:100 | Citra; steamer 30 min |
cMet (Ventana) | SP44/Cat#790-4430 | Ventana Medical Systems Tucson, AZ | Prediluted | CC1 30 min |
cMet (Leica) | 8F11/NCL-cMet | Leica Biosystems Newcastle, United Kingdom | 1:80 | Citra; steamer 30 min |
Ki67 | M1B-1/Cat#M7240 | DAKO Carpinteria, CA | 1:250 | Trilogy; water bath (95 °C 25 min) |
EGFR, epidermal growth factor receptor; HER, human epidermal growth factor receptor; cMet, hepatocyte growth factor receptor.