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. 2016 Dec 22;6:262. doi: 10.3389/fonc.2016.00262

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Copyright © 2016 Mori, Wildes, Takagi, Glunde and Bhujwalla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Protein levels of Chk-α, cPLA₂, PLD₁, and fatty acid synthase (FASN) determined by immunoblot assays of DU-145, PC-3, MCF-7, and MDA-MB-231 cells (marked C) and the tumor xenografts (marked T). (B) Protein levels of FASN in DU-145 and MDA-MB-231 cells (C) and the tumor xenografts (T) were obtained from a separate immunoblot assay with different exposure time. 50 µg (A) or 40 µg (B) of protein was loaded on a 10% reducing SDS-PAGE gel. GAPDH protein levels were used for equal loading assessment.