. 2016 Dec 22;7:1912. doi: 10.3389/fpls.2016.01912

Table 1.

Relative distribution of metabolites in Col-0 before and after cold acclimation.

		*Col-0, Relative Distribution: Mean* ± SD [%]**
		CHLOROPLAST			CYTOSOL			VACUOLE
METABOLITES		Non Acc	Acc	p	Non Acc	Acc	p	Non Acc	Acc	p
SUGARS/SUGAR ALCOHOLS	Fructose	17.2 ± 5.7	22.6 ± 4.5		29.6 ± 10.4	28.5 ± 3.7		53.2 ± 14.5	48.9 ± 5.3
	Galactinol	27.7 ± 8	22.1 ± 6.7		45.3 ± 8.4	35.7 ± 5.5	↓^*	27 ± 11.9	42.2 ± 6.3	↑^**
	Glucose	18.1 ± 4.1	23.7 ± 4.7	↑^*	28.9 ± 8.3	28.1 ± 3.7		53 ± 10.4	48.3 ± 6.2
	Melibiose	23.5 ± 15.4	38.2 ± 5	↑^*	21.8 ± 9.4	35.5 ± 2.8	↑^**	47.2 ± 32	26.3 ± 7
	myo-Inositol	40.7 ± 4.8	32.5 ± 6	↓^*	38.1 ± 2.6	39.1 ± 5		17 ± 2.4	28.5 ± 2.8	↑^***
	Raffinose	25.4 ± 6.5	19.7 ± 8.2		43.9 ± 9.3	32.7 ± 4.4	↓^*	26.8 ± 5	43.6 ± 6.5	↑^**
	Sucrose	29.7 ± 5.2	27.6 ± 5.2		47.2 ± 2.1	39.4 ± 3.2	↓^***	24.6 ± 6.1	33 ± 3.3	↑^**
	Threitol	19.9 ± 14.5	23 ± 14.4		27.5 ± 7.7	29.4 ± 15.4		53.8 ± 20.8	48.5 ± 26
ORGANIC ACIDS	2-Oxoglutarate	32.3 ± 1.8	26.4 ± 14.6		47.9 ± 9.1	39.3 ± 7.4		25.5 ± 2.8	25.1 ± 12.7
	Citrate	21.2 ± 10.3	20.3 ± 7.8		32.3 ± 10.1	31.9 ± 5.1		46.5 ± 12.3	47.8 ± 9.8
	Fumarate	19.1 ± 13.7	19.3 ± 6		28.7 ± 11.3	28.4 ± 5.1		52.3 ± 18.8	52.3 ± 5.8
	Gluconate	33.3 ± 23.8	34.1 ± 1.8		31.8 ± 2.1	34.1 ± 2.5		38.9 ± 30.8	30.7 ± 4.1
	Malate	23.7 ± 12	21.1 ± 8.9		34.3 ± 11	34.3 ± 5.4		42.1 ± 10.6	44.6 ± 10.8
	Oxaloacetate	38.3 ± 18.1	34.9 ± 5.1		41.9 ± 14.1	34.6 ± 3.5		19.8 ± 9.2	29.8 ± 6.1	↑^*
	Pyruvate	29.1 ± 5.2	33.3 ± 0	↑^*	30 ± 4.5	33.3 ± 0		41 ± 8.9	33.3 ± 0	↓^*
	Succinate	22.6 ± 12.3	21.2 ± 7.6		31.1 ± 2.8	28.3 ± 4.9		51 ± 8.6	53.7 ± 5.2
	Threonate	38.3 ± 4.2	35.9 ± 6.4		42.6 ± 5.6	37.6 ± 4.5		18.3 ± 1.8	26.5 ± 5.7	↑^*
AMINO ACIDS/POLYAMINES	Alanine	26.9 ± 12	33.2 ± 6.6		51.2 ± 6.2	38 ± 6.4	↓^**	27.2 ± 2.8	28.8 ± 5.2
	Asparagine	46.7 ± 1	31.3 ± 15.4		37.5 ± 5.7	26.4 ± 13.3		14.6 ± 1.2	22.8 ± 16.4
	Aspartate	45.1 ± 6.3	47.7 ± 4		36.2 ± 3.1	31.1 ± 4.1	↓^*	16.8 ± 10.1	22.9 ± 3.8
	Glutamate	44.4 ± 8.8	47.3 ± 1.1		38.8 ± 5.8	30.9 ± 4.4	↓^*	16.7 ± 8.9	22.1 ± 4
	Glutamine	39.8 ± 11.6	21.8 ± 8.4	↓^**	37.8 ± 4.8	33.7 ± 8.5		19.8 ± 9.9	44.5 ± 14	↑^**
	Glycine	22.6 ± 11.2	19.2 ± 4		37.1 ± 11.7	32.6 ± 7.7		40.3 ± 10.4	45.6 ± 10.1
	Isoleucine	26.1 ± 13.2	19.4 ± 4.5		47.7 ± 4.3	31.9 ± 7.7	↓^**	34.6 ± 6.4	46.6 ± 10.4	↑^*
	Leucine	27 ± 11.8	20.8 ± 3.8		43.4 ± 13	34 ± 5.6		29.6 ± 10	43.5 ± 8.1	↑^*
	Lysine	29.3 ± 6.3	18.6 ± 7.6	↓^*	41.3 ± 12.9	23.2 ± 10.2	↓^*	29.4 ± 11.4	58.2 ± 16.8	↑^**
	Methionine	38.4 ± 1.3	33.7 ± 6.1		43.2 ± 4.9	38 ± 4.1	↓^*	19.5 ± 7.2	28.9 ± 5.7	↑^*
	Ornithine	35.5 ± 3.9	40.2 ± 25.2		45.9 ± 6.9	29.9 ± 15	↓^*	18.5 ± 7.5	22 ± 12.9
	Phenylalanine	25.6 ± 10.1	18.5 ± 7.8		47.4 ± 8.4	26.7 ± 4.1	↓^***	32.6 ± 10.1	53.2 ± 9.2	↑^**
	Proline	27.6 ± 8.5	29 ± 2.6		47.1 ± 10.2	37.5 ± 4.9	↓^*	25.3 ± 9.3	31.6 ± 5.1
	Serine	28.9 ± 7.7	24.1 ± 2.8		47.7 ± 10.3	37.8 ± 5	↓^*	23.4 ± 8.7	35.7 ± 6.1	↑^**
	Threonine	30.6 ± 13	25.9 ± 3.9		45.5 ± 15.9	37.9 ± 5.8		20 ± 4.6	34.1 ± 9.4	↑^*
	Tryptophan	20.8 ± 8.5	30.9 ± 3.4	↑^**	30.1 ± 16	33.2 ± 0.4		49.1 ± 23.4	34.6 ± 2.5
	Tyrosine	25.4 ± 5.7	16.7 ± 10.5		38.9 ± 14.8	19.9 ± 10.9	↓^*	35.7 ± 14.4	64.2 ± 20.6	↑^*
	Valine	25.8 ± 12	19.6 ± 3.3		48.3 ± 4.2	32.5 ± 6.3	↓^***	30.4 ± 11.2	45.8 ± 9.4	↑^*
	Putrescine	38.2 ± 4	37.8 ± 7.7		40.5 ± 3.5	38 ± 5.3		19.1 ± 5	24.2 ± 3.5	↑^*
	Spermidine	46.7 ± 4.2	33.4 ± 0.1	↓^***	33.6 ± 5.7	33.5 ± 0.3		20.7 ± 1.3	32.6 ± 3.4	↑^***

Mean values of non-acclimated plants (Non Acc) and acclimated plants (Acc). (n ≥ 6); Asterisks indicate significant changes during acclimation per compartment (ANOVA and Tukey,

p < 0.05;

^**

p < 0.01;

^***

p < 0.001) and arrows (↑; ↓) indicate an increase or decrease.