Figure 1.

Δp Dissipation Triggers Selective Complex I Peripheral Arm Proteolysis

(A and B) SH-SY5Y and HeLa cells after 4-, 8-, and 18-hr CCCP treatment (n = 5).

(C and D) HSP60 and NDUFV2 immunostained HeLa cells ± 18-hr CCCP treatment (n = 3).

(E and F) Native-PAGE complex I, III, and IV levels, following 0- to 8-hr CCCP or 8-hr nigericin (Nig) or valinomycin (Val) (n = 3).

(G and H) Native-PAGE in-gel complex I activity in HeLa cells and quantification compared to citrate synthase activity ± 18-hr CCCP (^∗non-complex I reactive band; n = 3).

(I) Activity measurements in mitochondria isolated from HeLa cells ± CCCP (n = 3).

(J and K) Respiration rates in HeLa cells ± 8-hr CCCP or valinomycin pre-treatment. Oligomycin (Oligo), FCCP, and antimycin A + rotenone (Ant.A + Rot) were added as indicated (n = 4).

(L and M) Native-PAGE NDUFA9 and NDUFA10 immunoblots. HeLa cells ± 8- and 18-hr CCCP. All data are presented in the figure as mean + SDM.

See also Figure S1.