Figure 3.

Activating Mitophagy Induces Mitochondrial Oxidative Stress and Triggers Peripheral Arm Degradation

(A and B) Aconitase activity in HeLa cells after antimycin A (Anti.A) ± oligomycin (Oligo), rotenone (Rot) ± Oligo. Red zone indicates activity attributable to the cytosolic isoform. (n = 4–5).

(C) HeLa cells ± 8-hr CCCP ± NAC (n = 3).

(D and E) Anti.A-treated SH-SY5Y cells ± cycloheximide (CHX) (n = 3).

(F) mRNA levels in SH-SY5Y cells ± Anti.A treatment (n = 3).

(G and H) NAD(P)H autofluorescence (a.u.) in mitochondrial-GFP (mt-GFP)-expressing SH-SY5Y cells during Anti.A treatment (n = 3).

(I and J) Rates (a.u.) of ROS in mt-GFP SH-SY5Y cells incubated with mitoSOX and treated with Anti.A (n = 3).

(K) NADH-induced H₂O₂ production in mitochondria isolated from HeLa cells ± 8-hr CCCP (n = 3). All data in the figure are presented as mean + SDM.

See also Figure S2.