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. 2016 Dec 22;10:282. doi: 10.3389/fncel.2016.00282

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Copyright © 2016 Gu, Bao, Chen, Huang, Zhang, Jiang, Liu, Liu, Xu and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CaMKII enhances the expression of NP847 in NSCs. (A) Horizontal sections of adult rat brain hippocampus 3 days after TBI were labeled fo rCaMKII (red), Nestin (green, first and second line) and NP847 (green, third and fourth line) (small panels represent high magnification images). Scale bars: 100 μm. (B) Graphs (relative optical density) quantifying the intensity of (A). (C) Primary NSCs were treated with LPS (100 μM) or OGD for 12 h or transfected by LV-RNAi-CaMKII. Then, cell extracts were prepared, and protein expression was examined by western blotting. (D) Graphs (relative optical density) quantifying the intensity of (C). The values are presented as the mean ± SEM (n = 3). ^#*$P < 0.05 indicates significant differences compared to the sham group.