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. 2016 Dec 22;7:1947. doi: 10.3389/fpls.2016.01947

FIGURE 1.

FIGURE 1

SA-, MeJA-, ACC-, and their combinations-induced pathogen- and wound-responsive genes in Mediator subunit mutants. (A) SA-induced PR1, MeJA-induced VSP1 and PDF1.2, and ACC-induced PDF1.2 in 14 Mediator subunit mutants. Ten-day-old seedlings of Col-0 and the indicated Mediator mutants except med15 grown on ½12 × MS medium as well as 3-week-old soil-grown Col-0 and 7-week-old soil-grown med15 plants were treated with 0.5 mM SA, 0.1 mM MeJA, or 0.1 mM ACC. Plant tissues were collected 6 h after the treatment for analysis of VSP1 and 24 h for PR1 and PDF1.2. (B) SA-mediated inhibition of MeJA-induced expression of VSP1 in med14, med15, and med16. Ten-day-old Col-0, med14, and med16 seedlings grown on ½12 × MS medium as well as 3-week-old soil-grown Col-0 and 7-week-old soil-grown med15 plants were treated with 0.1 mM MeJA or 0.1 mM MeJA plus 0.5 mM SA. Plant tissues were collected 6 h after the treatment. (C) ET-mediated inhibition of MeJA-induced expression of VSP1 in med14 and med15. Ten-day-old Col-0 and med14 seedlings grown on ½12 × MS medium as well as 3-week-old soil-grown Col-0 and 7-week-old soil-grown med15 plants were treated with 0.1 mM MeJA or 0.1 mM MeJA plus 0.1 mM ACC. Plant tissues were collected 6 h after the treatment. (D) SA-induced PR gene expression in med25 in the presence and absence of MeJA. Ten-day-old Col-0 and med25 seedlings grown on ½12 × MS medium were treated with 0.5 mM SA or 0.5 mM SA plus 0.1 mM MeJA. Plant tissues were collected 24 h after the treatment. Total RNA was extracted from the collected plant tissues and subjected to real-time qPCR analysis. Expression of the target genes was normalized against the constitutively expressed UBQ5. Data represent means of three biological replicates with standard deviation (SD). Asterisks indicate that the induction of the gene was significantly lower or higher (A) and the inhibition of MeJA-induced VSP1 expression was significantly weaker (B,C) in the mutant than in the Col-0 plants (∗P < 0.05, ∗∗P < 0.01, two-way ANOVA).