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. 2016 Dec 22;6:39665. doi: 10.1038/srep39665

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The pSIP409-NP-M1-DCpep was generated as described in the Methods. NP: nucleo-protein gene; M1: matrix protein gene; DCpep: dendritic cell-targeting peptide; ermL: erythromycin-resistance marker; 256rep: replication origin for Lactobacillus; PorfX and PsppIP: inducible promoters; sppR and sppK: response regulator and histidine protein kinase, respectively. (b) Western blotting of the synthesized proteins. All cultures were induced with SppIP for 8 h and then subjected to an immunological assay using an anti-NP prime antibody. Lane 1: negative control, NC8(pSIP-409); lane 2: NC8(pSIP409-NP-M1-Ctrlpep), lane 3: NC8(pSIP409-NP-M1-DCpep); and lane 4: Purified NP protein.