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. 2016 Dec 22;6:39665. doi: 10.1038/srep39665

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Copyright © 2016, The Author(s)

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(a) The DC gating strategy. Mouse bone marrow DCs were co-cultured with recombinant L. plantarum for 12 h, and the expression levels of CD80 (b) and CD86 (c) were analysed by flow cytometry. (d) DCs were also isolated from the small intestines of mice immunized with recombinant L. plantarum or H9N2 inactivated vaccine for 24 h, and the expression levels of CD80, CD86, CD40 and MHC-II were determined. The results are presented as the means ± S.E.M of triplicate tests (n = 8–10 mice in each group), and were analysed by using a one-way ANOVA, assuming a Gaussian distribution, followed by the Bonferroni Post-test, and the statistical significance of differences between groups were analysed (*P < 0.05, **P < 0.01, and ***P < 0.001).