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. 2016 Dec 22;6:39737. doi: 10.1038/srep39737

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Copyright © 2016, The Author(s)

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(A) An SDS-PAGE gel stained with Coomassie blue. Lanes: (L) ladder, (1) crude E. coli lysate, (2) Ni-NTA eluate, (3) supernatant after heating for 2 hrs at 60 °C, (4) flow through from a Q HP anion exchange column. Yield was 1 mg purified P. torridus MBD from 32 L of culture. (B). P. torridus MBD (black) and R. castenholzii mevalonate 5-phosphate decarboxylase (blue) were assayed via our GC-FID decarboxylase assay after incubation with the following substrates for 1 hour: (MEV) mevalonate, (M3P) mevalonate 3-phosphate, (M5P) mevalonate 5-phosphate, (MBP) mevalonate 3,5-bisphosphate, (MPP) mevalonate 5-pyrophosphate and (NS) no substrate.