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. 2016 Dec 22;6:39693. doi: 10.1038/srep39693

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Cell viability ratio of E. coli with pET28a-LEA and pET28a (as control gourp) under heat treatment (A) and the initial colony number without heat treatment (B). (A) Cell viability ratio = (colony number on heated plate/colony number on unheated plate) × 100%. The procedure of heat treatment is shown in Figure 6S. The values are the mean ± SE of three samples. (B) The initial colony number of un-treated plates (without heat treatment), n = 3, means ± SE (one-way ANOVA, p > 0.05).