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. 2016 Dec 22;6:39714. doi: 10.1038/srep39714

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Copyright © 2016, The Author(s)

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WT and IL-13^−/− mice (n = 3–5 mice) were subjected to 5 × 6 Gy thoracic irradiation or maintained unirradiated (0 Gy). F4/80 and Arginase-1 expression were assessed in lung tissue sections collected at 16 weeks after irradiation (a–c). The number of stained cells per high power field (HPF, 40x) was scored in 6 fields per mouse. Lung tissue from IL-13^−/− mice demonstrated significantly reduced accumulation of cells expressing F4/80 and Arginase-1. (d) RT-PCR analysis of irradiated lung tissue demonstrated a significant reduction in radiation induced CCL2 expression in IL-13^−/− mice compared to WT lung tissue. Columns: mean, error bars: +SD, brackets: p < 0.05; ANOVA with Tukey’s correction.