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. 2016 Dec 22;6:38746. doi: 10.1038/srep38746

Figure 6. Chronic morphine treatment increases the MOR/D2DR interactions in the spinal cord dorsal horn and spinal D2DR inhibition with D2DR antagonist disrupts these interactions.

Figure 6

(A) Double immunofluorescence staining showed that MOR (green) and D2DR (red) were co-localized in the mice spinal cord (20X magnification). Chronic morphine treatment increased the co-localization of MOR and D2DR in the spinal cord, and D2DR antagonist sulpiride (8 μg/10 μl, i.t.) reduced the increased co-expression of D2DR with MOR (n = 4). (B) Co-IP experiments showed that D2DR could interact with MOR, and the MOR/D2DR interactions were increased in the spinal dorsal horn after chronic morphine treatment for 7 days while D2DR antagonist sulpiride (8 μg/10 μl, i.t.) disrupted the interactions of the MOR/D2DR (n = 3).