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. 2016 Dec 22;6:39838. doi: 10.1038/srep39838

Figure 5. Assessment of functional recovery in a murine hindlimb ischemia model.

Figure 5

(a) The murine hindlimb ischemia model was established through ligation of the proximal femoral artery and boundary vessels of 8-week-old male Balb/C nude mice. Improvements in blood perfusion were assessed by laser Doppler perfusion imaging analysis of the ischemic-injured tissues of mice injected with PBS, MSCs (MSC), TUDCA-treated MSCs (TUDCA), TUDCA-treated MSCs pretreated with PRNP-specific siRNA (TUDCA + siPRNP), and TUDCA-treated MSCs pretreated with scramble siRNA at 0 days, 3 days, 7 days, 14 days, 21 days, and 28 days postoperation. (b) The ratio of blood perfusion (blood flow in the left ischemic limb/blood flow in the right non-ischemic limb) was measured in each of the five groups. Values represent the mean ± SEM. *P < 0.05 and **P < 0.01 vs. PBS, ##P < 0.01 vs. TUDCA. (c) Representative images illustrating the different outcomes (foot necrosis, toe loss, and limb salvage) of ischemic limbs injected with five treatments at postoperative day 28. (d) Distribution of the outcomes in each group at postoperative day 28.