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. 2016 Dec 22;6:39505. doi: 10.1038/srep39505

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Copyright © 2016, The Author(s)

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Effect of different pCS on cleavability of the HA protein (A) and plaque sizes (B). Cleavability of the HA of recombinant viruses 6 hours post infection was determined by Western Blot analyses after inoculation of CEK cells at a MOI of 1 in the absence of trypsin (A). Cell-to-cell spread was assessed by measuring the plaque sizes in MDCKII cells 72 hours post infection. The mean plaques sizes of the parental viruses were adjusted to 100% and the plaque sizes of recombinant viruses were given in relation to them.