Table 2. Clinical examination and pathogenicity index (PI) of inoculated and contact birds.
| No. | Virus | Abbreviation | PI | Mortality (MDT; range of mortality per day after inoculation or after contact) |
Shedding at 4dpi (No. of positive/Total examined) |
||
|---|---|---|---|---|---|---|---|
| Infected | Contact | Oral | Cloacal | ||||
| 1 | H03 A/chicken/Germany/R28/2003 (H7N7) | H03 | 2.1 | 5/6 (4.2; 4–5) | 3/4 (5–7) | 4/6 | 4/6 |
| 2 | H03_CSIT | 2.3 | 6/6 (4.2; 3–5) | 4/4 (6–8) | 5/6 | 5/6 | |
| 3 | H03_CSUK | 1.4 | 3/6 (5.3; 5–6) | 1/4 (6) | 6/6 | 6/6 | |
| 4 | L01 A/turkey/Germany/R11/2001 (H7N7) | L01 | 0.3 | 0/6 (n.a.) | 0/4 (n.a.) | 3/6 | 0/6 |
| 5 | L01_CSGE | 1.2 | 2/6 (6.5; 4–9) | 2/4 (7–8) | 3/6 | 4/6 | |
| 6 | L01_CSIT | 1.8 | 4/6 (5.1; 3–5) | 2/4 (6–7) | 3/6 | 2/6 | |
| 7 | L01_CSUK | 0.9 | 2/6 (7.5; 5–10) | 0/4 (n.a.) | 1/6 | 1/6 | |
| 8 | L01_HA03 | 0.6 | 0/4 (n.a.) | 1/4 (6) | 4/6 | 2/6 | |
| 9 | H03_HA01CSGE | 2.4 | 6/6 (4.4; 3–5) | 4/4 (6–9) | 3/3 | 3/3 | |
| 10 | L11 A/chicken/Germany/R1361/2011 (H7N7) | L11 | 0.2 | 0/6 (n.a.) | 0/4 (n.a.) | 5/6 | 3/6 |
| 11 | L11_CSGE | 2.4 | 6/6 (4.2; 4–5) | 4/4 (7–8) | 5/5 | 5/5 | |
| 12 | L11_CSIT | 2.1 | 6/6 (5.3; 4–7) | 3/4 (7–8) | 6/6 | 6/6 | |
| 13 | L11_CSUK | 1.2 | 3/6 (5.7; 5–7) | 0/3 (n.a.) | 6/6 | 6/6 | |
| 14 | L11_HA03 | 0.4 | 0/4 (n.a.) | 0/4 (n.a.) | 3/3 | 3/3 | |
| 15 | H03_HA11CSGE | 2.5 | 6/6 (3.5; 3–4) | 2/4 (4) | 6/6 | 6/6 | |
| 16 | L13 A/turkey/Germany/R534/2013 (H7N7) | L13 | 0.2 | 0/6 (n.a.) | 0/4 (n.a.) | 4/6 | 2/6 |
| 17 | L13_CSGE | 2.5 | 6/6 (3; 3) | 4/4 (4–5) | 5/6 | 3/6 | |
| 18 | L13_CSIT | 1.9 | 5/6 (5.4; 5–7) | 3/4 (6–7) | 5/6 | 6/6 | |
| 19 | L13_CSUK | 1.4 | 3/6 (5; 5) | 0/4 (n.a.) | 5/6 | 5/6 | |
Viruses were arranged in the table according to the experimental settings in vivo and in vitro.
MDT = Mean death time, n.a. = not applicable.
The pathogenicity index (PI) was calculated for each group as the sum of the daily arithmetic mean values divided by 10; the number of observation days. Virus shedding in swab samples from inoculated chickens was estimated by a generic real-time reverse transcription polymerase chain reaction assay.