Figure 6. Effect of temperature on yeast growth.

Yeasts were first grown on liquid GYP medium for three days and then diluted to OD = 1. Serial of dilutions (OD = 10⁻², 10⁻³, 10⁻⁴, 10⁻⁵, 10⁻⁶, 10⁻⁷) were plated on GYP agar medium for grown at different temperature conditions (8 °C, 21 °C, 30 °C, 37 °C). Colony forming units (CFUs) were counted to quantify growth after seven days. No isolate grew at 37 °C. Similar results were observed from three independent biological replicate experiments.