Table 1. Cleavage and blastocyst development after supplementing hMMP9 at 18 h of oocyte maturation.
| Effect | No. PZs | Cleavage (69.94 ± 6.77 hpi) |
Blastocyst development (211.37 ± 1.25 hpi) |
|||
| % Cleaved of PZs |
% 8 to 16-cell of cleaved |
% Blastocysts of PZs |
Stage | Nuclei | ||
| Maturation temperature (ºC) | ||||||
| 38.5 | 1642 | 69.76A | 72.72A | 26.83 A | 7.22 | 140.71 |
| 41.0 | 1723 | 60.76B | 62.27B | 16.35 B | 7.14 | 136.23 |
| SEM | 1.7 | 3.1 | 2.0 | 0.05 | 5.4 | |
| P-value | < 0.0001 | < 0.0001 | < 0.0001 | 0.0926 | 0.3798 | |
| hMMP9 (ng/ml) | ||||||
| 0 (Diluent) | 1166 | 67.23 | 69.00 | 24.07 A | 7.15 | 131.24 |
| 30 | 1026 | 64.45 | 67.57 | 19.39 B | 7.20 | 143.07 |
| 300 | 1173 | 64.48 | 66.56 | 20.10 B | 7.18 | 141.09 |
| SEM | 1.9 | 3.3 | 2.1 | 0.05 | 6.0 | |
| P-value | 0.2916 | 0.6079 | 0.0220 | 0.6884 | 0.1334 | |
| Maturation temperature × hMMP9 | ||||||
| 38.5ºC × 0 ng/mL (Diluent) | 580 | 70.33 | 74.83 | 30.17 | 7.17 | 135.74 |
| 41.0ºC × 0 ng/ml (Diluent) | 586 | 63.97 | 62.49 | 18.86 | 7.14 | 126.75 |
| 38.5ºC × 30 ng/ml | 485 | 71.64 | 72.30 | 26.41 | 7.27 | 148.21 |
| 41.0ºC × 30 ng/ml | 541 | 56.55 | 62.45 | 13.88 | 7.13 | 137.93 |
| 38.5ºC × 300 ng/ml | 557 | 67.20 | 70.95 | 24.11 | 7.21 | 138.17 |
| 41.0ºC × 300 ng/ml | 596 | 61.65 | 61.85 | 16.62 | 7.15 | 144.00 |
| SEM | 2.4 | 3.7 | 2.4 | 0.07 | 7.4 | |
| P-value | 0.0513 | 0.7364 | 0.3189 | 0.5486 | 0.3544 | |
hMMP9 = human matrix metallopeptidase-9; hpi = hours post in vitro fertilization; PZs = Putative Zygotes. AB Least squares means within a column differ.