Table 2. Cleavage and blastocyst development after supplementing hMMP9 at 12 h of oocyte maturation.
| Effect | No. PZs | Cleavage (72.32 ± 0.62 hpi) |
Blastocyst development (210.68 ± 0.81 hpi) |
|||
| % Cleaved of PZs |
% 8 to 16-cell of cleaved |
% Blastocysts of PZs |
Stage | Nuclei | ||
| Maturation temperature (ºC) | ||||||
| 38.5 | 1677 | 66.77 | 59.56 A | 25.31 A | 7.16 | 137.65 |
| 41.0 | 1633 | 66.55 | 51.01 B | 19.74 B | 7.19 | 133.99 |
| SEM | 1.6 | 3.2 | 1.7 | 0.06 | 6.3 | |
| P-value | 0.8940 | 0.0006 | 0.0062 | 0.5886 | 0.4238 | |
| hMMP9 (ng/ml) | ||||||
| 0 (Diluent) | 1655 | 68.76 A | 56.90 | 24.12 | 7.15 | 139.37 |
| 300 | 1655 | 64.50 B | 53.74 | 20.77 | 7.20 | 132.27 |
| SEM | 1.6 | 3.2 | 1.7 | 0.06 | 6.3 | |
| P-value | 0.0157 | 0.1561 | 0.0823 | 0.4332 | 0.1200 | |
| Maturation temperature × hMMP9 | ||||||
| 38.5ºC × 0 ng/ml (Diluent) | 855 | 69.40 | 60.20 | 27.85 | 7.17 | 143.72 |
| 41.0ºC × 0 ng/ml (Diluent) | 800 | 68.11 | 53.53 | 20.75 | 7.13 | 135.02 |
| 38.5ºC × 300 ng/ml | 822 | 64.04 | 58.92 | 22.93 | 7.14 | 131.58 |
| 41.0ºC × 300 ng/ml | 833 | 64.95 | 48.48 | 18.77 | 7.26 | 132.96 |
| SEM | 2.0 | 3.5 | 2.2 | 0.08 | 7.0 | |
| P-value | 0.5054 | 0.4018 | 0.5318 | 0.2142 | 0.2638 | |
hMMP9 = human matrix metallopeptidase-9; hpi = hours post in vitro fertilization; PZs = Putative Zygotes. AB Least squares means within a column differ.