Table 3. Cleavage and blastocyst development after supplementing hMMP9 at 0 h of oocyte maturation.
| Effect | Cleavage (73.5 ± 1.6 hpi) |
Blastocyst development (211.2 ± 0.65 hpi) |
||||
| % Cleaved of PZs |
% 8 to 16-cell of cleaved |
% Blastocysts of PZs |
Stage | Nuclei | ||
| Maturation temperature (ºC) | ||||||
| 38.5 | 76.62 | 52.57 A | 26.86 A | 7.26 | 149.73 | |
| 41.0 | 72.64 | 40.86 B | 21.86 B | 7.12 | 122.92 | |
| SEM | 4.1 | 2.8 | 2.5 | 0.17 | 17.6 | |
| P-value | 0.2246 | 0.0246 | 0.0305 | 0.5142 | 0.3231 | |
| hMMP9 (ng/ml) | ||||||
| 0 (Diluent) | 74.69 | 46.91 | 25.77 | 7.30 | 147.52 | |
| 300 | 74.67 | 46.42 | 22.95 | 7.08 | 125.13 | |
| SEM | 4.1 | 2.8 | 2.5 | 0.17 | 17.6 | |
| P-value | 0.9949 | 0.9048 | 0.1633 | 0.3397 | 0.4034 | |
| Maturation temperature × hMMP9 | ||||||
| 38.5ºC × 0 ng/ml (Diluent) | 76.54 | 52.44 | 28.91 | 7.20 | 148.63 | |
| 41.0ºC × 0 ng/ml (Diluent) | 72.76 | 41.46 | 22.63 | 7.40 | 146.40 | |
| 38.5ºC × 300 ng/ml | 76.71 | 52.69 | 24.81 | 7.32 | 150.83 | |
| 41.0ºC × 300 ng/ml | 72.52 | 40.26 | 21.08 | 6.84 | 99.44 | |
| SEM | 4.6 | 3.9 | 2.8 | 0.23 | 24.9 | |
| P-value | 0.9469 | 0.8560 | 0.5014 | 0.1531 | 0.3618 | |
hMMP9 = human matrix metallopeptidase-9; hpi = hours post in vitro fertilization; PZs = Putative Zygotes. AB Least squares means within a column differ.