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. 2016 Aug 29;62(6):591–597. doi: 10.1262/jrd.2016-095

Fig. 2.

Fig. 2.

Screening for the most effective anti-Xist siRNA. A: Structural illustration of the porcine Xist gene showing the target site of the 3 designed anti-Xist siRNAs. B: Structural illustration of the plasmid expressing anti-Xist siRNA target sequences fused with the EGFP gene. The anti-Xist siRNA target site is about 400 bp, composed of partial sequences from exons 1, 5, and 6 of the pig Xist, and covers the target sequences of 3 designed anti-Xist siRNAs. C: Fluorescence microscopy analysis of EGFP expression, 48 h after co-transfection with anti-Xist siRNA target-EGFP fusion gene expression plasmid and siRNAs into HEK 293 cells. D: Flow cytometry of relative EGFP+ cell percentage, 48 h after co-transfection with the anti-Xist siRNA target-EGFP fusion gene expression plasmid and siRNAs into HEK 293 cells. E: Inhibition of Xist expression in female pig fibroblasts by 3 different siRNAs. ** The mean values, calculated from 3 replicates, are significantly different between the 2 groups (P < 0.01).