Fig. 3.

Inhibition of Xist expression at different pre-implantation stages of male pig SCNT embryos injected with anti-Xist siRNA1. Xist mRNA levels in each group were measured from a mixture of 10–20 embryos collected at the 4-cell (48 h post activation), 16-cell (96 h post activation), and morula (120 h post activation) stages. At each stage, Xist mRNA levels in the 5 µM and 50 µM siRNA1-injected groups were normalized to that in the non-injected (control) group, which was defined as 1. ** The mean values, calculated from 3 replicates, are significantly different between the 2 groups (P < 0.01).