Fig. 1.

Additional poly(A) tail extension by PAPOLB and its effect on mRNA metabolism. Pachytene spermatocytes (P) and round spermatids (R) of wild-type (+/+) and PAPOLB-null (–/–) mice were purified by unit gravity sedimentation on 2–4% BSA gradients. (A) Northern blot analysis. Total RNAs were incubated with RNase H in the presence [–Poly(A)] or absence [+Poly(A)] of oligo(dT)₁₅ and subjected to Northern blot analysis with the cDNA probes indicated. Hist1h1t and Prm2 were used as meiosis- and haploid-specific markers, respectively. Asterisks represent Prm2 mRNA derived from round spermatids contaminated during germ cell fractionation. (B) Immunoblot analysis. Protein extracts were analyzed by immunoblotting using the antibodies indicated. RPL26 was used as a control.