Figure 5. The effect of non-allelic flagellar genes mutations on swimming motility and flagellar production.

(A) Swimming motility assays of the flagellar gene deletion mutants. The results represent at least three independent experiments and the error bars indicate the standard deviation. (B) TEM analysis of WP3 flagellar gene mutants that were cultured in liquid media. The black arrow indicates the polar flagellum. The scale bars are indicated in the lower-left corner. (C) SDS-PAGE analysis of isolated flagellins from WP3 flagellar gene mutants. Flagellins from the same volume of log-phase cultures adjusted to the same optical density were extracted and separated using SDS-PAGE. The black arrow indicates the band representing the polar flagellin. The full-length gels are presented in Supplementary Figure S5.