Sampling of Crotalus adamanteus for venom pools. We collected venom samples from 46 adult C. adamanteus across six populations as well as from 13 juveniles from a single population. Population designations are consistent with those of Margres et al. (2015a; 2015b): SAP (Ca7, Sapelo Island, Georgia), ANF adult and juvenile (Ca2, Apalachicola National Forest and the surrounding region east of the Apalachicola River and west of the Suwannee River, Florida), LSG (Ca5, Little St. George Island, Florida), SH (Ca3, Spring Hill, Florida), CAL (Ca6, Caladesi Island, Florida), and ENP (Ca4, Everglades National Park, Florida). AR, Apalachicola River; SMR, Saint Mary’s River; SR, Suwannee River.