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Treatment with 0.5% O2 forced ∼50% of TGC differentiation from mTSCs by Day 6. A) The quantification of TGC percentage over 6 days of 0.5% O2 culture. B–D) Examples of the nucleus-staining image taken at Days 0 (B), 3 (C), and 6 (D). Bar in B = 200 μm. Asterisk (*) indicates where statistical significance was found compared with 0-day control (P < 0.05), and letter a indicates significantly higher TGC percentage at 4–6 days of 0.5% O2 exposure compared with 2–3 days.
