Table 2.
16S rRNA based analysis of the bacterial diversity within the microaerophilic enrichment culture ADE-12-1.
| 16S rRNA librarya | 16S-tag Illuminab | |
|---|---|---|
| –Total number of clones or sequence reads | 100 | 42,639 |
| –Most abundant taxa within ADE-12-1 and Gallionella | ||
| • Telmatospirillum | 14 (14%) | 19,680 (46.2%) |
| • Opitutus | 13 (13%) | 9261 (21.7%) |
| • Sideroxydans (total)c | 70 (70%) | 4133 (9.7%) |
| OTU_31d | – | 4131 (99.95%) |
| OTU_1160d | – | 1 (0.002%) |
| OTU_1869d | – | 1 (0.002%) |
| • Gallionellaceae | 70 (70%) | 4178 (9.8%) |
| Percentage Sideroxydans of Gallionellaceaec | 100% | 98.92% |
| • Gallionellac | 0 (0%) | 0 (0%) |
a
Number of clones screened by ARDRA with subsequent sequence analysis of representatives of each ARDRA group.
b
Numbers represent 16S-tag sequence reads.
c
Only those reads were considered that were assigned to genus level.
d
Of total Sideroxydans reads.