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. 2016 Dec 20;4:e2637. doi: 10.7717/peerj.2637

Table 3. Key morphological features of clinically isolated D. eschscholtzii.

Culture medium Macroscopic features Microscopic features
Sabouraud dextrose agar Colonies attain a diameter of 9 cm agar plate in 5 days of incubation at 30 °C.
Colonies are felty and azonate.
At first colonies are whitish, turning smoke gray with slight olivaceous tone in age.
Reverse appears black in color.
Hyphae are septate, thin-walled and hyaline to thick-walled and melanized, with thick-walled hyphae often have blackish exudates.
Conidiophores are septate, hyaline to melanized, some with blackish exudates, mononematously, dichotomously or trichotomously irregular branched, occasionally branched from conidiogenous region, bearing one to three conidiogenous cells on its terminus, up to 167 µm length × 2.2–3.3 µm diameter.
Conidiogenous cells are cylindrical and hyaline, bearing conidia on its apical region, 8.9–27.8 µm length × 1.1–2.2 µm diameter.
Conidia are ellipsoid, aseptate, solitary, hyaline, with attenuated base, produced holoblastically in sympodial sequence, 4.4–6.7 µm length × 1.7–2.2 µm diameter.
Potato dextrose agar Colonies attain a diameter of 9 cm agar plate in 5–7 days of incubation at 30 °C.
Colonies are felty, azonate or zonate.
At first colonies are whitish, turning smoke gray with slight olivaceous tone with age.
Reverse appears black in color.
V8 juice agar Colonies attain a diameter of 9 cm agar plate in 5 days of incubation at 30 °C.
Colonies are felty to fluffy, azonate.
At first colonies are whitish, later turning gray or black, some with slight olivaceous tone.
Reverse is initially uncolored, later becoming slight blackish.