Table 1.
Comparison of microparticle testing technologies.
| Technology | Principle | Manufacturer | Invasive1 | Standards, dilutions required | Trained specialist required | MP separation required | Prep. time [min] |
Time/test [min] | Daily maintenance |
|---|---|---|---|---|---|---|---|---|---|
| ThromboLUX | DLS | LightIntegra | No | No | No | No | 5 | 8 | No |
|
| |||||||||
| Flow cytometer | Static light scattering, fluorescence | Abbott, Becton Dickinson amongst others | No | Yes | Yes | Yes | 30+ | 5–10 | Yes |
|
| |||||||||
| Micro flow cytometer | Static light scattering, fluorescence | Apogee flow systems | No | Yes | Yes | Yes | 30+ | 5–10∗ | Yes |
|
| |||||||||
| qNano Gold | Size exclusion chromatography | Izon Science | Yes | Yes | Yes | Yes | 20† | 10–15 | No |
|
| |||||||||
| NanoSight | DLS particle tracking | Malvern | Yes | Yes | Yes | Yes | 15 | 6–75 | No |
|
| |||||||||
| ELISA | Double antibody sandwich technique | JIMRO Co. Ltd., Diagnostica Stago | No | Yes | Yes | Yes | 30+ | 5–10 | No |
1The test is considered invasive if the required sample volume is larger than what can be aseptically obtained from a tubing segment.
∗Consumables only usable for 6–8 hours. †Isolation of microparticles by differential centrifugation or size exclusion chromatography required, dynamic light scattering (DLS), microparticles (MP), and enzyme-linked immunosorbent assay (ELISA).