Table 2.
Cell cycle analysis after treatment with α-M, CDDP, and the combined treatments using both the coincubation and preincubation schemes. Values are the mean ± SD of three independent experiments for each point.
| Groups | G1/G0 | S | G2/M | G2/G1 ratio |
|---|---|---|---|---|
| Coincubation | ||||
| Control | 59.1 ± 6.3 | 13.3 ± 4.3 | 27.6 ± 4.8 | 0.48 ± 0.12 |
| CDDP 2 μM | 42.7 ± 8.3a | 23.3 ± 7.4a | 34.0 ± 5.8 | 0.83 ± 0.26 |
| α-M 10 μM | 62.4 ± 7.1 | 12.3 ± 2.8 | 25.3 ± 4.8 | 0.42 ± 0.13 |
| α-M 15 μM | 66.9 ± 6.3 | 10.6 ± 3.0 | 22.6 ± 5.8 | 0.35 ± 0.12 |
| CDDP+α-M (2/10 μM) | 42.8 ± 2.1a | 18.5 ± 4.2 | 38.7 ± 4.7a | 0.91 ± 0.14 |
| CDDP+α-M (2/15 μM) | 60.2 ± 8.7 | 13.8 ± 4.6 | 26.0 ± 5.9 | 0.45 ± 0.15 |
|
| ||||
| Preincubation | ||||
| Control | 51.5 ± 1.7 | 11.9 ± 1.5 | 36.6 ± 2.2 | 0.71 ± 0.06 |
| CDDP 2 μM | 29.8 ± 6.1a | 20.6 ± 4.9a | 53.8 ± 6.0a | 1.86 ± 0.41 |
| α-M 10 μM | 52.7 ± 7.3 | 12.0 ± 1.2 | 35.3 ± 3.8 | 0.70 ± 0.26 |
| α-M 15 μM | 50.1 ± 4.7 | 11.3 ± 3.1 | 38.7 ± 2.4 | 0.78 ± 0.11 |
| CDDP+α-M (2/10 μM) | 27.2 ± 5.8a | 22.5 ± 6.5a | 45.3 ± 7.2 | 1.73 ± 0.43 |
| CDDP+α-M (2/15 μM) | 40.1 ± 11.2a | 23.2 ± 5.1a | 38.8 ± 8.7 | 1.17 ± 0.63 |
a p ≤ 0.05 versus control group.