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. 2004 Oct;24(20):9198–9206. doi: 10.1128/MCB.24.20.9198-9206.2004

FIG. 3.

FIG. 3.

PAL-E-immunoprecipitated protein is recognized by the V9 antivimentin monoclonal antibody. A. Immunoblot analysis of PAL-E IP. HMEC-1 cell lysate was immunoprecipitated by using PAL-E-conjugated beads, and the precipitated proteins were analyzed using PAL-E, the V9 monoclonal antivimentin antibody, and the H84 polyclonal antivimentin antibody in the presence (+) or absence (−) of the reducing agent 2-mercaptoethanol. Note the detection of two bands of approximately 120 and 140 kDa in molecular mass by the V9 antibody. B. Immunoblot analysis of HMEC-1 cell lysate with PAL-E and antivimentin antibodies. The HMEC-1 cell lysate used for the IP in (A) was analyzed directly with PAL-E, V9, and H84 antibodies under nonreduced conditions. Note the preferential recognition of a 120-kDa protein by PAL-E and a 140-kDa protein by V9 and H84.