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PAL-E-reactive vimentin is not the product of an endothelial cell-specific mRNA. Vimentin-encoding mRNAs were analyzed in PAL-E+ V9+ HMEC-1 cells and PAL-E− V9+ HEK-293 cells by Northern blotting. The probe used to detect vimentin transcripts in the two cell lines corresponded to a region encompassing amino acids 65 to 310, a region defined as contained within the PAL-E antigen by the analysis shown in Fig. 2.
