TABLE 1.

Primers for real-time PCR assays

Genea	Species	Amplicon (bp)	Oligonucleotide
Collagen I*	Mouse	51	Forward primer: 5′-GTA TCT GCC ACA ATG GCA CG-3′
			Reverse primer: 5′-CTT CAT TGC ATT GCA CGT CAT-3′
BSP*	Mouse	51	Forward primer: 5′-GCA CTC CAA CTG CCC AAG A-3′
			Reverse primer: 5′-TTT TGG AGC CCT GCT TTC TG-3′
Osteopontin*	Mouse	51	Forward primer: 5′-TTT GCT TTT GCC TGT TTG C-3′
			Reverse primer: 5′-CAG TCA CTT TCA CCG GGA GG-3′
AP	Mouse	75	Forward primer: 5′-TTG TGC CAG AGA AAG AGA GAG A-3′
			Reverse primer: 5′-GTT TCA GGG CAT TTT TCA AGG T-3′
			Taqman probe: 5′-TAC TGG CGA CAG CAA G-3′
OC	Mouse	59	Forward primer: 5′-CTG ACA AAG CCT TCA TGT CCA A-3′
			Reverse primer: 5′-GCG GGC GAG TCT GTT CAC TA-3′
			Taqman probe: 5′-AGG AGG GCA ATA AG-3′

^a*, primer used for Sybr Green dye detection.